Oxygen derived free radicals have been implicated as important pathologic mediators in experimentally induced pancreatitis model. to assess the preventive effects of allopurinol, specific xanthine oxidase antagonist, on bile duct ligation induced
pancreatitis of rats, different doses of allopurinol (25㎎/㎏/day, 50㎎/㎏/day, 100㎎/㎏/day) were administrated per orally consecutive 5days in each group.
And serum amylase concentration, histologic grading of pancreatic injury, tissue malondialdehyde content were measured at 12 hours after bile duct ligation.
The results are as follows;
1) Amylase concentration was 6790±280 somogyi unit/dl in control group, 6888±242 unit/dl in allopurinol group with 25㎎/㎏/day. 6333±736 unit/dl in allopurinol group with 50㎎/㎏/day, 4766±300 unit/dl in allopurinol group with 100㎎/㎏/day,
(control vs 100㎎/㎏/day, p<0.005)
2) Pancreatic tissue demage was significantly attenuated in allopurinol group with 100㎎/㎏day compared with control group in aspects of edema, inflammation, acinar injury (p<0.05)
3) Malondialdehyde content of pancreatic tissue was 7.97±1.56, in control group, 8.05±1.83 in alloparinoi group with 25㎎/㎏/day, 7.42±1.49 in allopurinol group with 50㎎/㎏/day, 5.94±1.05 nM/100 mg of wet weight with 100㎎/㎏/day, p<0.05)
4) Thease data reveal that the pretrearment with high dose of allopurinol attenuates the pancteatic injury, and sugges+ that oxygen derived free radicals generated by xanthine oxidase may have an important role in the development of bile duct
ligation
induced pancreatitis of rats.
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